Journal: bioRxiv

Article Title: Development of Scaffold-free 3D Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis

doi: 10.1101/2022.10.24.513594

Figure Lengend Snippet: A . IF staining for Desmin (red), and αSMA (green) of immortalized human HSCs from normal and PSC patients. Right, quantification of Desmin + and αSMA + cell percentage (% Hoechst stained nucleus) in isolated normal and PSC HSCs (n=3 patients for normal and PSC) (* = p <0.05). B . IF staining for GFAP (red), and POSTN (green) of immortalized human HSCs from normal and PSC patients. Right, quantification of GFAP + and POSTN + cells (% Hoechst stained nucleus) in isolated normal and PSC HSCs (n=3 patients for normal and PSC) (* = p <0.05). C . IF staining for CD31 (green) of immortalized human LECs from normal and PSC patients. Hoechst (blue) staining for nucleus counterstain. Right, quantification of CD31 + cells (% Hoechst stained nucleus) in isolated normal and PSC LECs. Scale bars: 100µm. D . qRT-PCR analysis of the expression of Desmin and PECAM in HSCs and LECs from normal and PSC patients, confirming cell phenotypes and characteristics.

Article Snippet: The NPCs that remained in the supernatant were centrifuged at 600x g for 10 minutes at 4°C, and then resuspended to purify LECs with FACS to select CD31 + cells (Miltenyi Biotech, Bergisch Gladbach, Germany, Catalog No. Cat# 130-117-312).

Techniques: Staining, Isolation, Quantitative RT-PCR, Expressing

Journal: bioRxiv

Article Title: Development of Scaffold-free 3D Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis

doi: 10.1101/2022.10.24.513594

Figure Lengend Snippet: A . Representative brightfield images of 3-cell 3D-CHOs (cholangiocytes, LECs, HSCs) from normal and PSC patients on day 1 and day 4. A large number of cell aggregates (within the inner white line circle and outer layer circle) detached from the main organoid (within the inner circle) were observed in PSC organoids on day 4. B . CellTiterGlo 3D assay on normal and PSC 3D-CHOs showing significant proliferation in PSC cells after culturing for 5 days (n=3 per normal and PSC organoids). C . Top: representative IF co-staining image for CK-19 (in red for cholangiocytes) and αSMA (in green for HSCs) in normal and PSC organoids. Middle: representative IF co-staining image for CFTR (in green for cholangiocytes) and COL1A1 (in red for HSCs) in normal and PSC organoids. The side magnified image shows a nodular-like image in PSC organoids. Bottom: representative IF staining image for CD31 (in green for LEC vascularization) in normal and PSC organoids. Cell nucleus was stained with Hoechst (in blue). Scale bars: 100µm. D . qRT-PCRs for cholangiocyte marker genes Sox9, EpCAM, SCT (secretin) and SCTR (secretin receptor), fibrosis marker genes ACTA2, COL1A1, DESMIN (DES), and TGFβ1, angiogenesis marker genes PECAM, CDH5, and vWF, and inflammation marker genes IL-6 and TNFα. *P<0.05, **p<0.01 PSC vs. normal organoids. (n=4 organoids per each experiment).

Article Snippet: The NPCs that remained in the supernatant were centrifuged at 600x g for 10 minutes at 4°C, and then resuspended to purify LECs with FACS to select CD31 + cells (Miltenyi Biotech, Bergisch Gladbach, Germany, Catalog No. Cat# 130-117-312).

Techniques: Staining, Marker

Journal: Immunity, inflammation and disease

Article Title: Expression of Major Basic Protein and Endothelial Adhesion Molecules in Chronically Inflamed Mucosa of the Ethmoid Labyrinth.

doi: 10.1002/iid3.70066

Figure Lengend Snippet: FIGURE 1 | Examples of immunohistochemical staining in the concha bullosa (CB) mucosa (top row) and nasal polyps (NPs) (bottom row). A1, BMK‐13 expression in the CB mucosa; A2, CD31 expression in the CB mucosa; A3, CD34 expression in the CB mucosa; B1, BMK‐13 expression in NPs; B2, CD31 expression in NPs; B3, CD34 expression in NPs. (Magnification ×100). On slide A1, we can see zero degrees, and on slide B1, we can see degree 4 of immunoreactivity for BMK‐13. On slide A2, we can see the 1st degree, and on slide B2, we can see degree 3 of immunoexpression for CD31. On slide A3, we can see the 2nd degree, and on slide B3, we can see degree 3 of immunoreactivity for CD34.

Article Snippet: Human BMK‐13 antibody (Santa Cruz Biotechnology, Inc. Dallas, Texas, USA) was used to detect MBP‐positive eosinophils, while human anti‐CD31 and anti‐CD34 antibodies (Elabscience, Houston, Texas, USA) were used to detect CD31 and CD34 molecules.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

doi: 10.1002/jcsm.12974

Figure Lengend Snippet: Antibody panel used for CyTOF

Article Snippet: CD31 , 144Nd , WM59 , Fluidigm , 3144023B.

Techniques:

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD31 , 145Nd , Fluidigm , 3145004B , AB_2737262.

Techniques: Mass Cytometry

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: CyTOF single-cell analysis of surface antigens in PDHGG patient-derived cell lines. t-SNE maps showing the expression of 10 surface markers (CD31, CD34, CD63, CD36, CD29, CD90, CD140a, CD49c, CD56 and CD61) in each of the eight different PDHGG patient-derived cell lines analyzed through mass cytometry technique. The color gradient refers to the intensity of the expression of the considered marker, in a blue to red scale indicating low and high intensity respectively.

Article Snippet: Anti-Human CD31 , 145Nd , Fluidigm , 3145004B , AB_2737262.

Techniques: Single-cell Analysis, Derivative Assay, Expressing, Mass Cytometry, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: List of the cell antigens used together with the molecular function and reported expression.

Article Snippet: Anti-Human CD31 , 145Nd , Fluidigm , 3145004B , AB_2737262.

Techniques: Expressing, Migration, Mutagenesis